Hydrolysis of synthetic pyrophosphoric esters by an isoenzyme of apyrase from Solanum tuberosum.

@article{Campo1977HydrolysisOS,
  title={Hydrolysis of synthetic pyrophosphoric esters by an isoenzyme of apyrase from Solanum tuberosum.},
  author={G B Del Campo and Javier P{\'e}rez Puente and Mar{\'i}a Antonieta Valenzuela and A{\'i}da Traverso-Cori and Osvaldo Cori},
  journal={The Biochemical journal},
  year={1977},
  volume={167 3},
  pages={
          525-9
        }
}
A highly purified isoenzyme of apyrase obtained from potatoes (Solanum tuberosum var. Pimpernel) exhibits a low specificity for the organic moiety of synthetic pyro- and triphosphates. Methyl di- and tri-phosphates were hydrolysed at higher rates than ADP and ATP, but their Km values were also higher. Steric hindrance at the carbon atom linked to the pyrophosphate chain decreases both binding and maximum rate, whereas length or polarity of the organic chain do not have systematic effects. t… CONTINUE READING