Hydrolysis of proteins by immobilized-stabilized alcalase-glyoxyl agarose.

@article{Tardioli2003HydrolysisOP,
  title={Hydrolysis of proteins by immobilized-stabilized alcalase-glyoxyl agarose.},
  author={Paulo Waldir Tardioli and Justo Pedroche and Raquel Lima Camargo Giordano and Roberto Fernandez-Lafuente and Jose M Guisan},
  journal={Biotechnology progress},
  year={2003},
  volume={19 2},
  pages={
          352-60
        }
}
This paper presents stable Alcalase-glyoxyl derivatives, to be used in the controlled hydrolysis of proteins. They were produced by immobilizing-stabilizing Alcalase on cross-linked 10% agarose beads, using low and high activation grades of the support and different immobilization times. The Alcalase glyoxyl derivatives were compared to other agarose derivatives, prepared using glutaraldehyde and CNBr as activation reactants. The performance of derivatives in the hydrolysis of casein was also… CONTINUE READING
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