Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin.

@article{Blanchoin2002HydrolysisOA,
  title={Hydrolysis of ATP by polymerized actin depends on the bound divalent cation but not profilin.},
  author={Laurent Blanchoin and Thomas D. Pollard},
  journal={Biochemistry},
  year={2002},
  volume={41 2},
  pages={597-602}
}
Growing evidence suggests that the nucleotide bound to actin filaments serves as a timer to control actin filament turnover during cell motility (Pollard, T. D., Blanchoin, L., and Mullins, R. D. (2000) Annu. Rev. Biophys. Biomol. Struct. 29, 545-576). We re-examined the hydrolysis of ATP by polymerized actin using mechanical quenched-flow methods to improve temporal resolution. The rate constant for ATP hydrolysis by polymerized Mg actin is 0.3 s(-1), 3-fold faster than that measured manually… CONTINUE READING