Hydrolases in Vacuoles from Castor Bean

Abstract

Vacuoles were prepared from endosperm tissue of 4-day-old castor bean seedlings (Ricinus communis var. Hale) and purified on a stepped sucrose gradient. It was shown by assays of marker enzymes that there was only trace contamination of the final preparation by other organeiles (mitochondria, glyoxysomes, nuclei, spherosomes, and plastids) and by… (More)

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