Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.

@article{Nakagawara1981HydrogenPM,
  title={Hydrogen peroxide metabolism in human monocytes during differentiation in vitro.},
  author={Akira Nakagawara and Carl Nathan and Zanvil Alexander Cohn},
  journal={The Journal of clinical investigation},
  year={1981},
  volume={68 5},
  pages={1243-52}
}
The capacity of human blood monocytes to secrete hydrogen peroxide (H2O2) and superoxide (O2-) was measured as the cells differentiated during 4 wk of culture. Morphologic transformation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells accompanied a steady increase in the content of protein per cell, from 0.77 mg/10(7) cells on days 0 to 11.77 mg/10(7) cells on days 20 to 29. In contrast, secretion of H2O2 by adherent monocytes was 859 +/- 73 nmol/60 min per mg… CONTINUE READING