Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general mechanism.

@article{Kirsch1998HydrogenPF,
  title={Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general mechanism.},
  author={Michael Kirsch and Elena E Lomonosova and Hans Gert Korth and Reiner Sustmann and Herbert de Groot},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 21},
  pages={12716-24}
}
Organic amine-based buffer compounds such as HEPES (Good's buffers) are commonly applied in experimental systems, including those where the biological effects of peroxynitrite are studied. In such studies 3-morpholinosydnonimine N-ethylcarbamide (SIN-1), a compound that simultaneously releases nitric oxide (.NO) and superoxide (O-2), is often used as a source for peroxynitrite. Whereas in mere phosphate buffer H2O2 formation from 1.5 mM SIN-1 was low ( approximately 15 microM), incubation of… CONTINUE READING