Hydrogen peroxide and the proliferation of BHK-21 cells.

@article{Burdon1995HydrogenPA,
  title={Hydrogen peroxide and the proliferation of BHK-21 cells.},
  author={Roy H. Burdon and Dmi Alliangana and Vera Gill},
  journal={Free radical research},
  year={1995},
  volume={23 5},
  pages={
          471-86
        }
}
Intracellular levels of H2O2 in BHK-21 cells are not static but decline progressively with cell growth. Exposure of cells to inhibitors of catalase, or glutathione peroxidase, not only diminishes this decline but also depresses rates of cell proliferation, suggesting important growth regulatory roles for those antioxidant enzymes. Other agents which also diminish the growth-associated decline in intracellular levels of H2O2, such as the superoxide dismutase mimic, copper II-(3,5… 

Hydrogen peroxide in relation to proliferation and apoptosis in BHK-21 hamster fibroblasts.

Addition of H2O2 at 100 microM, or 1 mM, to the culture medium of BHK-21 fibroblasts results in increased intracellular levels of H2O2. Whilst exposure of BHK-21 cells to lower levels of H2O2 (1

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It is concluded that G6PDH plays a critical role in cell death by affecting the redox potential by causing changes in mitogen-activated protein kinase phosphorylation that were similar to the changes seen with H2O2.

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The results support that acute oxidative stress caused by H2O2 inhibits the expression levels of NADH-cytochrome b5 reductase, most likely due to inhibition of SP1 transcriptional activity.

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This review evaluates the evidence that H2O2 functions as a signaling agent in higher organisms in light of the known biology and biochemistry of H2 O2 and identifies oxidation-dependent steps in signal transduction pathways being uncovered.

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Evidence is provided of a novel means of regulating antioxidants of the selenoprotein family via the mTOR pathway, which is dependent on the expression of Bcr-Abl and its activity regulates the translation of several proteins.
...

References

SHOWING 1-10 OF 32 REFERENCES

Oxidative stress and tumour cell proliferation.

TLDR
The effects of oxidant stress were studied in immortalised hamster and rat cell lines before and after transformation to the malignant state with polyoma virus, or activated H-ras, respectively and the possible role of active oxygen species in the promotion of cell growth was examined.

Endogenously generated active oxygen species and cellular glutathione levels in relation to BHK-21 cell proliferation.

TLDR
During the normal growth phase cellular levels of GSH actually decline although this effect can be partly reversed by N-acetylcysteine and by mercaptosuccinate which also impair proliferation of these cells.

Effects of 4-hydroxynonenal, a product of lipid peroxidation, on cell proliferation and ornithine decarboxylase activity.

TLDR
Data indicate a reduction of proliferative capacity of the cells and are consistent with the possibility that HNE, at concentrations close to those found in normal cells, plays a role in the control of cell proliferation.

Reduction of a tetrazolium salt and superoxide generation in human tumor cells (HeLa).

TLDR
It is suggested that at least 20-30% of the intracellular reduction of MTT is due to superoxide, and whilst a small proportion (30%) may arise through an NADPH-oxidase type enzyme, other sources of extracellular superoxide in HeLa cells remain a possibility.

α-Tocopherol Uptake and Its Influence on Cell Proliferation and Lipid Peroxidation in Transformed and Nontransformed Baby Hamster Kidney Cells

TLDR
The results demonstrate that, in this cell system, the growth-stimulating ability of α-T is unrelated to the ability ofα-T to control lipid peroxidation and that the level ofperoxidation is increased in the malignant state.

4-Hydroxynonenal, a product of cellular lipid peroxidation, which modulates c-myc and globin gene expression in K562 erythroleukemic cells.

TLDR
It appears that the restoration in human erythroleukemic K562 cells of HNE concentrations closer to the level in normal cells can modulate the expression of specific genes.

Effect of 4-Hydroxynonenal on c-myc Expression

TLDR
Preliminary results may suggest that the inhibition of c-myc expression is related to nucleic acid synthesis inhibition following HNE exposure.

Is there a significant role for lipid peroxidation in the causation of malignancy and for antioxidants in cancer prevention?

TLDR
The results demonstrate that, in this cell system, the growth-stimulating ability of alpha-T is unrelated to the ability ofalpha-T to control lipid peroxidation and that the level ofperoxidation is increased in the malignant state.