Hydrogen exchange in proteins as a measure of solvent exclusion due to ligands. Nuclease and myoglobin.


The kinetic distribution of hydrogen atoms back-exchanging from tritium-labeled proteins has been estimated from the results of interrupted flow and slow, continuousflow gel filtration. Staphylococcal nuclease exchanges almost all of it bound tritium atoms in a few hours at pH 8.1, but in the presence of calcium ions and deoxythymidine 3’,5’-diphosphate… (More)

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