Hydrogen exchange and the dynamic structure of proteins

  title={Hydrogen exchange and the dynamic structure of proteins},
  author={C. Woodward and I. Simon and E. T{\"u}chsen},
  journal={Molecular and Cellular Biochemistry},
  • C. Woodward, I. Simon, E. Tüchsen
  • Published 2004
  • Chemistry, Medicine
  • Molecular and Cellular Biochemistry
  • SummaryIn native proteins, buried, labile protons undergo isotope exchange with solvent hydrogens, but the kinetics of exchange are markedly slower than in unfolded polypeptides. This indicates that, whereas buried protein atoms are shielded from solvent, the protein fluctuates around the time average structure and occasionally exposes buried sites to solvent. Generally, hydrogen exchange studies are designed to characterize the nature of the fluctuations between conformational substates, to… CONTINUE READING

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