Hydrogen Ion Regulation in Rat Cerebellar Granule Cells Studied by Single-Cell Fluorescence Microscopy.


The internal pH (pHi) regulatory mechanisms of individual rat cerebellar granule cells maintained in tissue culture have been investigated using the fluorescent indicator BCECF (2,7' bis carboxyethyl 5,6 carboxy-fluorescein) and quantitative fluorescence microscopy. The steady-state pHi was estimated as 7.27 +/- 0.25 in bicarbonate-buffered media and 7.49… (More)


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