Hydrogel-encapsulated 3D microwell array for neuronal differentiation.

@article{Bae2016Hydrogelencapsulated3M,
  title={Hydrogel-encapsulated 3D microwell array for neuronal differentiation.},
  author={Jun Hyuk Bae and Jong Min Lee and Bong Geun Chung},
  journal={Biomedical materials},
  year={2016},
  volume={11 1},
  pages={
          015019
        }
}
We developed a photo-crosslinkable hydrogel-encapsulated three-dimensional (3D) microwell array for studying embryonic stem (ES) cell-derived neuronal differentiation. ES cells were cultured for 5 d in microwells and were subsequently encapsulated by photo-crosslinkable gelatin methacrylate (GelMA) and polyethylene glycol (PEG) hydrogels for an additional 7 d. We observed that ES cells cultured in PEG microwells became uniform-sized embryoid bodies (EBs) compared to those in GelMA microwells… CONTINUE READING
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