Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates

@article{Tanase2015HydrodynamicSS,
  title={Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates},
  author={Maya Tanase and Valerio Zolla and Cristina C. Clement and Francesco Borghi and Aleksandra Malgorzata Urbanska and Jos{\'e} Antonio Rodr{\'i}guez-Navarro and Barbara Roda and Andrea Zattoni and Pierluigi Reschiglian and Ana Mar{\'i}a Cuervo and Laura Santambrogio},
  journal={Nature Protocols},
  year={2015},
  volume={10},
  pages={134-148}
}
Herein we describe a protocol that uses hollow-fiber flow field-flow fractionation (FFF) coupled with multiangle light scattering (MALS) for hydrodynamic size-based separation and characterization of complex protein aggregates. The fractionation method, which requires 1.5 h to run, was successfully modified from the analysis of protein aggregates, as found in simple protein mixtures, to complex aggregates, as found in total cell lysates. In contrast to other related methods (filter assay… CONTINUE READING