Hydrodynamic effects on BHK cells grown as suspended natural aggregates.

@article{Moreira1995HydrodynamicEO,
  title={Hydrodynamic effects on BHK cells grown as suspended natural aggregates.},
  author={J. L. Moreira and Paula M. Alves and John G. Auniņ{\vs} and Manuel Jos{\'e} Teixeira Carrondo},
  journal={Biotechnology and bioengineering},
  year={1995},
  volume={46 4},
  pages={
          351-60
        }
}
Baby hamster kidney (BHK) cell aggregates grown in stirred vessels with different working volumes and impeller sizes were characterized. Using batch cultures, the range of agitation rates studied (25-100 rpm) led to aggregates with maximum sizes of 150 mum. Necrotic centers were not observed and cell specific productivity was independent of aggregate size. High cell viability was found for both single and adherent cells without an increase in cell death when agitation rate was increased. The… CONTINUE READING

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