Hydrodynamic Effects on Biofilms at the Biointerface Using a Microfluidic Electrochemical Cell: Case Study of Pseudomonas sp.

@article{Zarabadi2017HydrodynamicEO,
  title={Hydrodynamic Effects on Biofilms at the Biointerface Using a Microfluidic Electrochemical Cell: Case Study of Pseudomonas sp.},
  author={Mir Pouyan Zarabadi and François Paquet-Mercier and Steve J. Charette and Jesse Greener},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2017},
  volume={33 8},
  pages={
          2041-2049
        }
}
The anchoring biofilm layer is expected to exhibit a different response to environmental stresses than for portions in the bulk, due to the protection from other strata and the proximity to the attachment surface. The effect of hydrodynamic stress on surface-adhered biofilm layers was tested using a specially designed microfluidic bio flow cell with an embedded three-electrode detection system. In situ electrochemical impedance spectroscopy (EIS) measurements of biocapacitance and bioresistance… 

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References

SHOWING 1-10 OF 49 REFERENCES
Biofilms’ Role in Planktonic Cell Proliferation
TLDR
The existence of a biofilm surface-associated zone of planktonic cell proliferation is proposed to highlight the need to expand the traditional perception of bioFilms as promoting microbial survival, to include the potential of biofilms to contribute to microbial proliferation.
Influence of electric fields and pH on biofilm structure as related to the bioelectric effect
TLDR
The consequences of the explanation of the increased susceptibility of biofilm cells to antibiotics in an electric field, the "bioelectric effect," are discussed.
Effect of flow regime on the architecture of a Pseudomonas fluorescens biofilm.
TLDR
These conclusions, together with the fact that the biofilms were more dense and stable when formed in contact with turbulent flows, favor the design of more compact and efficient biofilm reactors operating in turbulent conditions.
Monitoring of microbial adhesion and biofilm growth using electrochemical impedancemetry
TLDR
The applicability of electric cell-substrate impedance sensing (ECIS) for studying the attachment and spreading of cells on a metal surface has been demonstrated and provides a simple and non-expensive electrochemical method for in vitro assessment of the presence of biofilms on metal surfaces.
Influence of hydrodynamics and nutrients on biofilm structure
Hydrodynamic conditions control two interlinked parameters; mass transfer and drag, and will, therefore, significantly influence many of the processes involved in biofilm development. The goal of
A microfluidic platform with pH imaging for chemical and hydrodynamic stimulation of intact oral biofilms.
TLDR
A microfluidic platform with a fluorescent nanoparticle-based sensor is demonstrated for real-time, ratiometric pH imaging of biofilms and opens the way for the analysis of separate contributions to dental caries due to localized acidification directly at the biofilm tooth interface.
Metabolic Differentiation in Biofilms as Indicated by Carbon Dioxide Production Rates
TLDR
The adaptable nature and the ability of bacteria to respond to environmental conditions were further demonstrated by the outer shear-susceptible region of the biofilm; the average CO2 production rate of cells from this region increased within 0.25 h from 9.45 ± 5.40 fmol of CO2·cell−1·h−1 to 22.6 ± 7.58 fmol when cells were removed from the bio film and maintained in suspension without an additional nutrient supply.
Mechanism and kinetics of biofilm growth process influenced by shear stress in sewers.
TLDR
A kinetic model for describing the development of sewer biofilms was developed and demonstrated to be capable of reproducing all the experimental data.
Micropatterned biofilm formations by laminar flow-templating.
TLDR
The design considerations and fabrication methodology of a two level flow-templating micro-bioreactor (FT-μBR), which generates a biofilm growth stream surrounded on 3 sides by a growth inhibiting confinement stream, are described.
...
1
2
3
4
5
...