Hybridoma perfusion systems: a comparison study.

  title={Hybridoma perfusion systems: a comparison study.},
  author={D de la Broise and M Noiseux and Bernard Massie and R{\'e}al Lemieux},
  journal={Biotechnology and bioengineering},
  volume={40 1},
The efficiency of lgM production by hybridoma cells (1) cultured in suspension; (2) entrapped in alginate beads; or (3) packed in hollowfiber cartridge bioreactors, were compared in long-term perfusion cultures. The results showed that steady-state cell concentration and antibody production, per liter of perfused medium per day, were similar when cells were either entrapped in alginate beads of maintained in suspension. These values were also similar whether cells were maintained at high… CONTINUE READING