Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

@article{Thomas1980HybridizationOD,
  title={Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.},
  author={Paul S. Thomas},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1980},
  volume={77 9},
  pages={
          5201-5
        }
}
  • P. Thomas
  • Published 1 September 1980
  • Biology, Chemistry
  • Proceedings of the National Academy of Sciences of the United States of America
A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed. Poly(A)+ and ribosomal RNAs transfer efficiently to nitrocellulose paper in high salt (3 M NaCl/0.3 M trisodium citrate) after denaturation with glyoxal and 50% (vol/vol) dimethyl sulfoxide. RNA also binds to nitrocellulose after treatment with methylmercuric hydroxide. The method is sensitive: about 50 pg of specific mRNA per band is readily detectable after… 

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