Hybridization kinetics and thermodynamics of molecular beacons.

  title={Hybridization kinetics and thermodynamics of molecular beacons.},
  author={Andrew Tsourkas and Mark A Behlke and Scott D. Rose and Gang Bao},
  journal={Nucleic acids research},
  volume={31 4},
Molecular beacons are increasingly being used in many applications involving nucleic acid detection and quantification. The stem-loop structure of molecular beacons provides a competing reaction for probe-target hybridization that serves to increase probe specificity, which is particularly useful when single-base discrimination is desired. To fully realize the potential of molecular beacons, it is necessary to optimize their structure. Here we report a systematic study of the thermodynamic and… CONTINUE READING
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