Hybridisoelectric focusing in rehydrated immobilized pH gradients with added carrier ampholytes: Demonstration of human globins

@inproceedings{Altland1985HybridisoelectricFI,
  title={Hybridisoelectric focusing in rehydrated immobilized pH gradients with added carrier ampholytes: Demonstration of human globins},
  author={Klaus Altland and Ute Rossmann},
  year={1985}
}
In contrast to hitherto described procedures, gels containing immobilized pH gradients were prepared from buffered solutions of constant, near neutral pH at ambient temperature. The washed, dried and rehydrated gels were run in the presence or absence of free carrier ampholytes, 8 M urea, Triton X-100, 2-mercaptoethanol and dithiothreitol, respectively. The patterns of human globins were compared for linear and modified pH gradients in the range between pH 6 and 10. By adding free carrier… CONTINUE READING