Hybrid surface platform for the simultaneous detection of proteins and DNAs using a surface plasmon resonance imaging sensor.

Abstract

In this work, we present a novel surface and assay for the simultaneous detection of DNA and protein analytes on a surface plasmon resonance (SPR) imaging sensor. A mixed DNA/oligo (ethylene glycol) (OEG) self-assembled monolayer (SAM) is created using a microarrayer. Thiol-modified single-stranded DNA sequences are spotted onto a gold-coated glass substrate. Backfilling with an OEG-modified alkanethiol creates a protein-resistant surface background. Antibodies conjugated to complementary single-stranded DNA sequences are immobilized on the surface through DNA hybridization. By converting only part of the DNA array into a protein array, simultaneous detections of DNA and protein analytes are possible. A model system of two cDNA sequences and two human pregnancy hormones are used to demonstrate the assay. No cross-reactivity was observed between DNA or protein analytes and nontargeted immobilized cDNA sequence or antibodies. A response from a detection of a single analyte in a mixture of protein and DNA analytes corresponds well with that of a single-analyte solution.

DOI: 10.1021/ac800263j

Cite this paper

@article{Ladd2008HybridSP, title={Hybrid surface platform for the simultaneous detection of proteins and DNAs using a surface plasmon resonance imaging sensor.}, author={Jon J. Ladd and Allen D. Taylor and Marek Piliarik and Jiri Homola and Shaoyi Jiang}, journal={Analytical chemistry}, year={2008}, volume={80 11}, pages={4231-6} }