Hybrid lethality of cultured cells of an interspecific F1 hybrid of Nicotiana gossei Domin and N. tabacum L.

@article{Mino2005HybridLO,
  title={Hybrid lethality of cultured cells of an interspecific F1 hybrid of Nicotiana gossei Domin and N. tabacum L.},
  author={Masanobu Mino and Yuko Misaka and Junko Ueda and Ken’ichi Ogawa and Masayoshi Inoue},
  journal={Plant Cell Reports},
  year={2005},
  volume={24},
  pages={179-188}
}
Cultured cells were established from the hypocotyl of F1 hybrid seedlings of Nicotiana gossei Domin and N. tabacum L. The cultured cells started to die at 26°C, but not at 37°C, which is similar to what occurred in cells of the original hybrid plants. An increase in the number of cells without cytoplasmic strands and acidification of the cytoplasm followed by decomposition of the mitochondria and chloroplasts indicated that vacuolar collapse plays a central role in the execution of cell death… CONTINUE READING