Hybrid error correction and de novo assembly of single-molecule sequencing reads

@inproceedings{Koren2012HybridEC,
  title={Hybrid error correction and de novo assembly of single-molecule sequencing reads},
  author={Sergey Koren and Michael C. Schatz and Brian P. Walenz and Jeffrey A. Martin and Jason T Howard and Ganeshkumar Ganapathy and Zhong Wang and David A. Rasko and W. Richard McCombie and Erich D. Jarvis and Adam M. Phillippy},
  booktitle={Nature Biotechnology},
  year={2012}
}
Single-molecule sequencing instruments can generate multikilobase sequences with the potential to greatly improve genome and transcriptome assembly. However, the error rates of single-molecule reads are high, which has limited their use thus far to resequencing bacteria. To address this limitation, we introduce a correction algorithm and assembly strategy that uses short, high-fidelity sequences to correct the error in single-molecule sequences. We demonstrate the utility of this approach on… CONTINUE READING
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