Hybrid Living Materials: Digital Design and Fabrication of 3D Multimaterial Structures with Programmable Biohybrid Surfaces

@article{Smith2019HybridLM,
  title={Hybrid Living Materials: Digital Design and Fabrication of 3D Multimaterial Structures with Programmable Biohybrid Surfaces},
  author={Rachel Soo Hoo Smith and Christoph Bader and Sunanda Sharma and Dominik Kolb and Tzu-Chieh Tang and Ahmed Hosny and Felix Moser and James C. Weaver and Christopher A. Voigt and Neri Oxman},
  journal={Advanced Functional Materials},
  year={2019},
  volume={30}
}
  • R. SmithC. Bader N. Oxman
  • Published 18 December 2019
  • Biology, Engineering, Materials Science
  • Advanced Functional Materials
Significant efforts exist to develop living/non‐living composite materials—known as biohybrids—that can support and control the functionality of biological agents. To enable the production of broadly applicable biohybrid materials, new tools are required to improve replicability, scalability, and control. Here, the Hybrid Living Material (HLM) fabrication platform is presented, which integrates computational design, additive manufacturing, and synthetic biology to achieve replicable fabrication… 
View on Wiley
dspace.mit.edu
39 Citations
Highly Influential Citations
2
Background Citations
7
Methods Citations
3

39 Citations

Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers

This report showcases the advanced capabilities of nanobiotechnology and living materials technology to 3D-print functional living architectures by embedding programmed Escherichia coli cells and nanofibers into microbial ink, which can sequester toxic moieties, release biologics and regulate its own cell growth through the chemical induction of rationally designed genetic circuits.

Recent Advances in 3D Printing with Protein-Based Inks.

Digitally Fabricated and Naturally Augmented In Vitro Tissues

This report presents advances in computational modeling of bioink polymerization and its compatibility with bioprinting, the use of digital design and fabrication in the development of fluidic culture devices, and the employment of generative algorithms for modeling the natural and biological augmentation of in vitro tissues.

Digitally Fabricated and Naturally Augmented In Vitro Tissues.

This report presents advances in computational modeling of bioink polymerization and its compatibility with bioprinting, the use of digital design and fabrication in the development of fluidic culture devices, and the employment of generative algorithms for modeling the natural and biological augmentation of in vitro tissues.

Hylozoic by Design: Converging Material and Biological Complexities for Cell‐Driven Living Materials with 4D Behaviors

The emergence of novel techniques in biology and material science, coupled with greater understandings of cell–material interactions, have given rise to the creation of living materials and…

Engineered Living Materials-Based Sensing and Actuation

This short review briefly reviewed the important recent development in ELMs-based sensors and actuators, with a focus on their materials and structural design, new fabrication technologies, and bio-related applications.

Design and biofabrication of bacterial living materials with robust and multiplexed biosensing capabilities

Bioprinting of Regenerative Photosynthetic Living Materials

A bioprinting technique employing environmentally friendly chemistry to encapsulate microalgae within an alginate hydrogel matrix is reported, highlighting their potential product applications including artificial leaves, photosynthetic bio‐garments, and adhesive labels.

Self-Assembled Biohybrid: A Living Material To Bridge the Functions between Electronics and Multilevel Biological Modules/Systems.

Exoelectrogens are known to be specialized in reducing various extracellular electron acceptors to form conductive nanomaterials that are integrated with their cell bodies both structurally and…

Materials design by synthetic biology

This Review discusses how synthetic-biology tools can be applied for the engineering of self-organizing functional materials and programmable hybrid living materials.

References

SHOWING 1-10 OF 87 REFERENCES

3D printing of bacteria into functional complex materials

3D printing of bacteria-laden hydrogels enables the digital fabrication of complex functional materials displaying spatially specific compositions, geometry, and properties not accessed by standard technologies can be assembled from bottom up for new biotechnological and biomedical applications.

Stretchable living materials and devices with hydrogel–elastomer hybrids hosting programmed cells

The design of a set of living materials and devices based on stretchable, robust, and biocompatible hydrogel–elastomer hybrids that host various types of genetically engineered bacterial cells are reported.

3D Printing of Living Responsive Materials and Devices

A new method and material system capable of 3D-printing hydrogel inks with programed bacterial cells as responsive components into large-scale, high-resolution living materials, where the cells can communicate and process signals in a programmable manner are reported.

Light‐Controlled, High‐Resolution Patterning of Living Engineered Bacteria Onto Textiles, Ceramics, and Plastic

An approach is presented to use light to pattern Escherichia coli onto diverse materials by controlling the expression of curli fibers that anchor the formation of a biofilm.

Programmable and printable Bacillus subtilis biofilms as engineered living materials

A highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis is described that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties.

Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials

Early efforts toward engineered living materials (ELMs) are reviewed, with an emphasis on engineered bacterial systems, living composite materials which integrate inorganic components, successful examples of large-scale implementation, and production methods.

A Straightforward Approach for 3D Bacterial Printing

This methodology combines the capability of bacteria to form new materials with the reproducibility and tailored approach of 3D printing systems to enable a sustainable approach for the production of numerous new materials.

Composite Living Fibers for Creating Tissue Constructs Using Textile Techniques

Composite living fibers in which a core of load bearing synthetic polymer is coated by a hydrogel layer containing cells or microparticles is introduced, demonstrating the feasibility of using these processes for engineering functional 3D tissue constructs.

Synthesis and patterning of tunable multiscale materials with engineered cells

It is shown that E. coli cells can organize self-assembling amyloid fibrils across multiple length scales, producing amyloids-based materials that are either externally controllable or undergo autonomous patterning.

3D bioprinting of tissues and organs

3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
...