Hyb-Seq: Combining target enrichment and genome skimming for plant phylogenomics1

@inproceedings{Weitemier2014HybSeqCT,
  title={Hyb-Seq: Combining target enrichment and genome skimming for plant phylogenomics1},
  author={Kevin Weitemier and Shannon C. K. Straub and Richard Cronn and Mark Fishbein and Roswitha E Schmickl and Angela McDonnell and Aaron Liston},
  booktitle={Applications in plant sciences},
  year={2014}
}
UNLABELLED PREMISE OF THE STUDY Hyb-Seq, the combination of target enrichment and genome skimming, allows simultaneous data collection for low-copy nuclear genes and high-copy genomic targets for plant systematics and evolution studies. • METHODS AND RESULTS Genome and transcriptome assemblies for milkweed (Asclepias syriaca) were used to design enrichment probes for 3385 exons from 768 genes (>1.6 Mbp) followed by Illumina sequencing of enriched libraries. Hyb-Seq of 12 individuals (10… CONTINUE READING