Hyaluronidase activity of human Hyal1 requires active site acidic and tyrosine residues.

@article{Zhang2009HyaluronidaseAO,
  title={Hyaluronidase activity of human Hyal1 requires active site acidic and tyrosine residues.},
  author={Ling Zhang and Alamelu G. Bharadwaj and Andrew J. Casper and Joel Barkley and Joseph J. Barycki and Melanie A. Simpson},
  journal={The Journal of biological chemistry},
  year={2009},
  volume={284 14},
  pages={
          9433-42
        }
}
Hyaluronidases are a family of endolytic glycoside hydrolases that cleave the beta1-4 linkage between N-acetylglucosamine and glucuronic acid in hyaluronan polymers via a substrate-assisted mechanism. In humans, turnover of hyaluronan by this enzyme family is critical for normal extracellular matrix remodeling. However, elevated expression of the Hyal1 isozyme accelerates tumor growth and metastatic progression. In this study, we used structural information, site-directed mutagenesis, and… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-2 OF 2 REFERENCES

Recent Advances in Carbohydrate Bioengineering (Gilbert,H

  • P. M. Coutinho, B. Henrissat
  • 1999
VIEW 1 EXCERPT

Ciba Found

  • D. C. West, S. Kumar
  • 1989