Hyaluronate tetrasaccharide- Cu(II) interaction: a NMR study.

Abstract

The coordination of Cu(II) to a hyaluronate tetrasaccharide (HAt) was investigated in aqueous solution by 13C and 1H relaxation measurements at two magnetic fields, 9 and 14 T. The HAt interaction with the metal ion was monitored following the nuclear paramagnetic relaxation enhancements R1p and R2p produced by the copper addition. The data analysis shows that the paramagnetic effect is differently experienced by the nuclei in different monosaccharide residues. A molecular model for the complex HAt-Cu(II) was built taking into account the experimental data. The model shows the presence of two binding sites, both involving the carboxylate groups of the two glucuronic acid units. The first site, that best simulates the HA binding site, is located on the ligand core, while the second one is located on the terminal glucuronic acid residue. Both binding sites involve, in addition to the carboxylate groups, the O4 oxygens of the glucuronic acid residues.

Cite this paper

@article{Dauria2003HyaluronateTC, title={Hyaluronate tetrasaccharide- Cu(II) interaction: a NMR study.}, author={G D'auria and Germana Flores and Lucia Falcigno and Romina Oliva and Manuela Vacatello and Maria Michela Corsaro and Michelangelo Parrilli and Livio Paolillo}, journal={Biopolymers}, year={2003}, volume={70 2}, pages={260-9} }