Hunting down fungal secretomes using liquid-phase IEF prior to high resolution 2-DE.


The secreted proteins (secretome) of fungi play a key role in interactions of pathogenic and symbiotic fungi with plants. Using the plant pathogenic fungus Leptosphaeria maculans and symbiont Laccaria bicolor grown in culture, we have established a proteomic protocol for extraction, concentration and resolution of the fungal secretome. As no proteomic data… (More)
DOI: 10.1002/elps.200900415



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