Hunter syndrome: presence of material cross-reacting with antibodies against iduronate sulfatase

@article{Daniele2004HunterSP,
  title={Hunter syndrome: presence of material cross-reacting with antibodies against iduronate sulfatase},
  author={A. Daniele and P. Natale},
  journal={Human Genetics},
  year={2004},
  volume={75},
  pages={234-238}
}
  • A. Daniele, P. Natale
  • Published 2004
  • Biology, Medicine
  • Human Genetics
  • SummaryPolyclonal antibodies were obtained from rabbits by injection of iduronate sulfatase purified 35,000-fold from human placenta, after elution of the enzyme from sodium dodecyl sulfate (SDS) polyacrylamide gels. The specificity of these antibodies towards iduronate sulfatase was demonstrated by immunoprecipitation of enzyme activity; the level of other lysosomal hydrolases and sulfatases remained constant. Immunoblot of iduronate sulfatase from various human sources showed that the… CONTINUE READING
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