Human zinc fingers as building blocks in the construction of artificial transcription factors

@article{Bae2003HumanZF,
  title={Human zinc fingers as building blocks in the construction of artificial transcription factors},
  author={K H Bae and Young Do Kwon and Hyun-Chul Shin and Moon-Sun Hwang and Eun-Hyun Ryu and Kyung-Soon Park and Hyo-Young Yang and Dong-ki Lee and Yangsoon Lee and Jinwoo Park and Heung Sun Kwon and Hyun-Won Kim and B. de Cambray T - S. Yeh and H W Lee and Soon Hyung Sohn and Joonho Yoon and Wongi Seol and Jin-Soo Kim},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={275-280}
}
We describe methods for generating artificial transcription factors capable of up- or downregulating the expression of genes whose promoter regions contain the target DNA sequences. To accomplish this, we screened zinc fingers derived from sequences in the human genome and isolated 56 zinc fingers with diverse DNA-binding specificities. We used these zinc fingers as modular building blocks in the construction of novel, sequence-specific DNA-binding proteins. Fusion of these zinc-finger proteins… CONTINUE READING

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exogenous PPARγ2 but not PPARγ1 reactivates adipogenesis

  • D. Ren, T. N. Collingwood, E. J. Rebar, A. P. Wolffe, Camp, H. S. PPARγ knockdown by engineered transcription factors
  • Genes Dev. 16, 27–32
  • 2002

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