Human zinc fingers as building blocks in the construction of artificial transcription factors

@article{Bae2003HumanZF,
  title={Human zinc fingers as building blocks in the construction of artificial transcription factors},
  author={Kwang-Hee Bae and Young Do Kwon and Hyun-Chul Shin and Moon Sun Hwang and Eun-Hyun Ryu and Kyung-Soon Park and Hyo-Young Yang and Dong-ki Lee and Yangsoon Lee and Jinwoo Park and Heung Sun Kwon and Hyun-Won Kim and Byung Il Yeh and Hyean-Woo Lee and Soon Hyung Sohn and Joonho Yoon and Wongi Seol and Jin-Soo Kim},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={275-280}
}
We describe methods for generating artificial transcription factors capable of up- or downregulating the expression of genes whose promoter regions contain the target DNA sequences. To accomplish this, we screened zinc fingers derived from sequences in the human genome and isolated 56 zinc fingers with diverse DNA-binding specificities. We used these zinc fingers as modular building blocks in the construction of novel, sequence-specific DNA-binding proteins. Fusion of these zinc-finger proteins… 
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The results show that randomized ZFP‐TF libraries are useful tools for improving the yield of heterologous recombinant protein both in yeast and mammalian cells.
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TLDR
Several applications of ATF library screenings in gene discovery, biotechnology, and disease therapeutics are summarized.
Construction of combinatorial libraries that encode zinc finger-based transcription factors.
TLDR
Here, protocols are provided for the preparation of retroviral libraries that encode ZF-TFs for use in mammalian cells that can activate or repress the transcription of endogenous target genes.
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TLDR
Direct in vivo selection of constituents of a zinc finger protein library may be an efficient method for isolating multi-finger DNA binding proteins while avoiding the context effect.
Design of a zinc finger protein binding a sequence upstream of the A20 gene
TLDR
It is feasible to design specific zinc finger proteins by bioinformatics methods and the ZFP designed successfully and exhibited biological activity in the A20 gene promoter region.
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