Human transforming growth factor. Production by a melanoma cell line, purification, and initial characterization.

@article{Marquardt1982HumanTG,
  title={Human transforming growth factor. Production by a melanoma cell line, purification, and initial characterization.},
  author={Hans Marquardt and George J. Todaro},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 9},
  pages={5220-5}
}
A low molecular weight human transforming growth factor (hTGFs) was isolated from serum-free medium conditioned by a human metastatic melanoma tumor line, A2058. The purification of hTGFs was achieved by gel permeation chromatography of the acid-soluble growth-promoting activity on Bio-Gel P-10 in 1 M acetic acid, followed by reverse phase high pressure… CONTINUE READING