Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence.

@article{Liu2007HumanSB,
  title={Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence.},
  author={De Yi Liu and Hugh William Gordon Baker},
  journal={Human reproduction},
  year={2007},
  volume={22 6},
  pages={1597-602}
}
BACKGROUND The aim of this study was to determine if only sperm with double stranded DNA could bind to the human zona pellucida (ZP). METHODS Sperm samples from 124 infertile men with a range of semen abnormalities were studied. Oocytes that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile sperm selected by colloidal silica gradient centrifugation (CSGC). After assessing the number of sperm… CONTINUE READING