Human skeletal muscle cells in ex vivo gene therapy to deliver bone morphogenetic protein-2.

@article{Musgrave2002HumanSM,
  title={Human skeletal muscle cells in ex vivo gene therapy to deliver bone morphogenetic protein-2.},
  author={Douglas S. Musgrave and Ryan Pruchnic and Pablo Bosch and Bruce H. Ziran and J Whalen and Johnny Huard},
  journal={The Journal of bone and joint surgery. British volume},
  year={2002},
  volume={84 1},
  pages={120-7}
}
We have examined whether primary human muscle-derived cells can be used in ex vivo gene therapy to deliver BMP-2 and to produce bone in vivo. Two in vitro experiments and one in vivo experiment were used to determine the osteocompetence and BMP-2 secretion capacity of cells isolated from human skeletal muscle. We isolated five different populations of primary muscle cells from human skeletal muscle in three patients. In the first in vitro experiment, production of alkaline phosphatase by the… CONTINUE READING