Human serum leads to differentiation of human hepatoma cells, restoration of very-low-density lipoprotein secretion, and a 1000-fold increase in HCV Japanese fulminant hepatitis type 1 titers.

@article{Steenbergen2013HumanSL,
  title={Human serum leads to differentiation of human hepatoma cells, restoration of very-low-density lipoprotein secretion, and a 1000-fold increase in HCV Japanese fulminant hepatitis type 1 titers.},
  author={Rineke Steenbergen and Michael A. S. Joyce and Bradley S Thomas and Daniel P. Jones and John Law and Rodney S Russell and Michael Houghton and D. Lorne Tyrrell},
  journal={Hepatology},
  year={2013},
  volume={58 6},
  pages={1907-17}
}
UNLABELLED In this study, we differentiated the human hepatoma cell line Huh7.5 by supplementing tissue culture media with human serum (HS) and examined the production of hepatitis C virus (HCV) by these cells. We compared the standard tissue culture protocol, using media supplemented with 10% fetal bovine serum (FBS), to media supplemented with 2% HS. Cells cultured in HS undergo rapid growth arrest, have a hepatocyte-like morphology, and increase the expression of hepatocyte differentiation… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 1 time over the past 90 days. VIEW TWEETS
17 Extracted Citations
26 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 17 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 26 references

Similar Papers

Loading similar papers…