Human serum albumin binding assay based on displacement of a non selective fluorescent inhibitor.

@article{Thorarensen2007HumanSA,
  title={Human serum albumin binding assay based on displacement of a non selective fluorescent inhibitor.},
  author={Atli Thorarensen and Ronald W. Sarver and Fang Tian and Andrea Ho and Donna Lee Romero and Keith R. Marotti},
  journal={Bioorganic & medicinal chemistry letters},
  year={2007},
  volume={17 16},
  pages={
          4646-9
        }
}
In this paper, we describe a fluorescent antibacterial analog, 6, with utility as a competition probe to determine affinities of other antibacterial analogs for human serum albumin (HSA). Analog 6 bound to HSA with an affinity of 400+/-100 nM and the fluorescence was environmentally sensitive. With 370 nm excitation, environmental sensitivity was indicated by a quenching of the 530 nm emission when the probe bound to HSA. Displacement of dansylsarcosine from HSA by 6 indicated it competed with… CONTINUE READING