Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor.

@article{Hanna1997HumanPK,
  title={Human pyridoxal kinase. cDNA cloning, expression, and modulation by ligands of the benzodiazepine receptor.},
  author={Michael C. Hanna and Anthony J. Turner and Ewen F. Kirkness},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 16},
  pages={10756-60}
}
Peptide fragments of a porcine benzodiazepine-binding protein were used to isolate the cDNA of a related human protein. The cDNA encodes a polypeptide of 312 amino acid residues that is homologous to a bacterial pyridoxal kinase. Transient expression of the cDNA in human embryonic kidney cells confirmed that it encodes human pyridoxal kinase. The recombinant enzyme displayed a Km value of 3.3 microM for pyridoxal and was inhibited competitively by 4-deoxypyridoxine (Ki = 2.8 microM… CONTINUE READING
17 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…