Human proinsulin conversion in the regulated and the constitutive pathways of transfected AtT20 cells.

@article{Irminger1994HumanPC,
  title={Human proinsulin conversion in the regulated and the constitutive pathways of transfected AtT20 cells.},
  author={J. C. Irminger and F M Vollenweider and M Neerman-Arbez and Philippe A. Halban},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 3},
  pages={1756-62}
}
AtT20 (mouse pituitary corticotroph) cells were stably transfected with human proinsulin cDNA. Clone H12 displayed low basal release of insulin-like immunoreactivity (< 1% cell content/30 min) with 17-fold stimulation by isobutylmethylxanthine/forskolin. Clone H23, by contrast, showed higher basal release (2.8% cell content/30 min) and only 6-fold stimulation. To follow the kinetics of conversion and release of only newly synthesized proinsulin, cells were pulse-chased, and labeled proinsulin… CONTINUE READING