Human platelet phenolsulfotransferases: cDNA cloning, stable expression in V79 cells and identification of a novel allelic variant of the phenol-sulfating form.

@article{Jones1995HumanPP,
  title={Human platelet phenolsulfotransferases: cDNA cloning, stable expression in V79 cells and identification of a novel allelic variant of the phenol-sulfating form.},
  author={Abigail L Jones and Michael Hagen and Michael W. H. Coughtrie and Ronald C Roberts and Hansruedi Glatt},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={208 2},
  pages={855-62}
}
We have isolated cDNA clones encoding phenol- and monoamine-sulfating phenolsulfotransferases, using the reverse transcription-polymerase chain reaction method with human platelet mRNA as template. These cDNAs were stably expressed in the Chinese hamster fibroblast cell line V79 and their substrate specificities towards known sulfotransferase isoenzyme-selective compounds determined. The nucleotide and derived amino acid sequences of the monoamine-sulfating form were identical to those… CONTINUE READING