Human plasma beta-endorphin-like peptides: a rapid, high recovery extraction technique and validation of radioimmunoassay.

@article{Cahill1983HumanPB,
  title={Human plasma beta-endorphin-like peptides: a rapid, high recovery extraction technique and validation of radioimmunoassay.},
  author={Cheryl Cahill and John D. Matthews and Huda Akil},
  journal={The Journal of clinical endocrinology and metabolism},
  year={1983},
  volume={56 5},
  pages={992-7}
}
This is a report of the development, calibration, and validation of a series of techniques required to measure beta-endorphin (beta-END)-like immunoreactivity in human plasma, including sieve and affinity chromatography. The RIA, which uses the antibody Brenda, is very sensitive (IC50 = 5-15 fmol/tube at a final concentration of 1:40,000). The extraction… CONTINUE READING