Human peripheral lymphocytes as indicators of microsomal epoxide hydrolase activity in liver and lung.

@article{Omiecinski1993HumanPL,
  title={Human peripheral lymphocytes as indicators of microsomal epoxide hydrolase activity in liver and lung.},
  author={Curtis John Omiecinski and Lauri Aicher and Richard Holubkov and Harvey Checkoway},
  journal={Pharmacogenetics},
  year={1993},
  volume={3 3},
  pages={150-8}
}
In this study, we have applied an improved assay for the determination of microsomal epoxide hydrolase activity to assess enzymatic levels in human lung, liver, and blood lymphocytes. The assay is fluorescence-based and monitors the epoxide hydrolase-mediated conversion of (+/-)-benzo[a]pyrene-4,5-epoxide to (+/-)-trans-benzo[a]pyrene-4,5-dihydrodiol, using a high pressure liquid chromatography separation system. Approximately a 40-fold range in microsomal epoxide hydrolase activities was… CONTINUE READING