Human peripheral blood granulocytes and myeloid leukemic cell lines express both transcripts encoding for stem cell factor

  title={Human peripheral blood granulocytes and myeloid leukemic cell lines express both transcripts encoding for stem cell factor},
  author={Ugo Ramenghi and Luciano Ruggieri and Irma Dianzani and C. Rossoxs and Maria Felice Brizzi and Clara Camaschella and Torsten Pietsch and Giuseppe Saglio},
  journal={STEM CELLS},
Stem cell factor (SCF), the ligand for the c‐kit proto‐oncogene, has been shown to play a critical role in the migration of melanocytes and germ cells during embryogenesis as well as in the proliferative control of the hematopoietic compartment. In this study we investigated the expression of both the soluble and transmembrane SCF forms in purified peripheral blood populations and in several hematopoietic cell lines. Expression of both transcripts, though in different ratios, was identified in… 
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KITLG (KIT ligand)
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It is suggested that SCF may be an important factor for the growth of myeloid leukemia cells, either as a direct stimulus or as a synergistic factor for other cytokines.
Expression of stem cell factor and c-kit mRNA in cultured endothelial cells, monocytes and cloned human bone marrow stromal cells (CFU-RF).
The view that the behaviour of proliferating erythroid stem cells in the marrow, which may express c-kit, could be regulated by membrane-bound SCF present on surrounding stromal cells is supported.
Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture
The derivation from myeloid leukaemic cells of a leukocyte culture is described here for the first time that by morphological and histochemical criteria clearly and persistently differentiates along the myeloids series without an exogenous source of conditioned medium.
Coexpression of the stem cell factor and the c-kit genes in small-cell lung cancer.
It is revealed that the human SCF gene is transcribed into two major forms of alternatively spliced mRNAs with different molar ratio in fetal, adult and malignant tissues.
Granulocyte-macrophage colony-stimulating factor induces cytokine secretion by human polymorphonuclear leukocytes.
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Expression and functional role of the proto-oncogene c-kit in acute myeloblastic leukemia cells.
Results indicate that c-kit proto-oncogene is expressed in most cases of AML and is functional in terms of supporting proliferation.
Soluble stem cell factor in human serum.
The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length, which suggest functional importance for soluble SCF in humans.
Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)
The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity of the cells, and it was concluded that the U‐937 is a neoplastic, histuocytic cell line.
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This CML cell-line represents a unique source of CML cells with meaningful indicators of malignancy for clinical and experimental studies.
M‐07e human leukemic factor‐dependent cell line provides a rapid and sensitive bioassay for the human cytokines GM‐CSF and IL‐3
A subline of the M‐07 human megakaryoblastic leukemia cell line that requires either interleukin‐3 (IL‐3) or granulocyte macrophage colony‐stimulating factor (GM‐CSF) for growth, even in the presence of fetal calf serum is isolated to develop a quantitative bioassay for the measurement of levels of either IL‐3 or GM‐ CSF.