[Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area].

Abstract

BACKGROUND The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. AIM To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. MATERIAL AND METHODS Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. RESULTS HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. CONCLUSIONS HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types with high oncogenic risk and included in commercial vaccines, were found in 25.1% and 47.1% of LSIL and HSIL, respectively.

Cite this paper

@article{M2010HumanPG, title={[Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area].}, author={Jaime L{\'o}pez M and Carmen Gloria Ili G and Priscilla Brebi M and Patricia Garc{\'i}a M and Italo Capurro V and Pablo Guzm{\'a}n G and Eugenio Su{\'a}rez P and Jos{\'e} Manuel Ojeda F and Juan Carlos Roa S}, journal={Revista médica de Chile}, year={2010}, volume={138 11}, pages={1343-50} }