Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors

  title={Human papillomavirus DNA detected in peripheral blood samples from healthy Australian male blood donors},
  author={Alice Che-Ha Chen and Annie Keleher and Mary Anne Kedda and Amanda B. Spurdle and Nigel A. J. McMillan and Annika Antonsson},
  journal={Journal of Medical Virology},
Recent studies have shown that human papillomavirus (HPV) DNA can be found in circulating blood, including peripheral blood mononuclear cells (PBMCs), sera, plasma, and arterial cord blood. In light of these findings, DNA extracted from PBMCs from healthy blood donors were examined in order to determine how common HPV DNA is in blood of healthy individuals. Blood samples were collected from 180 healthy male blood donors (18–76 years old) through the Australian Red Cross Blood Services. Genomic… 
Detection and quantitation of human papillomavirus DNA in peripheral blood mononuclear cells from blood donors
Results show that HPV DNA is present in PBMC from healthy blood donors and it suggests that blood could be a new route of HPV dissemination.
Detection and Analysis of Human Papillomavirus (HPV) DNA in Breast Cancer Patients by an Effective Method of HPV Capture
This study not only provided an efficient method to capture HPV DNA, but detected the SNVS, coverage, SNV type and depth, and has provided further clue of association between HPV16 and breast cancer.
Human papillomavirus proteins are found in peripheral blood and semen Cd20+ and Cd56+ cells during Hpv-16 semen infection
Considering the role of CD20+ and CD56+ cell populations in the antiviral immune response, the detection of HPV markers on leukocytes may reflect the presence of virus particles within the endosomal compartment.
Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia
Results obtained in this study confirm that HPV DNA can be detected and quantified in plasma samples of women with asymptomatic cervical infection.
Plasma HPV DNA is detectable in oral leukoplakia patients.
Elevation of HPV-18 and HPV-16 DNA in the plasma of patients with advanced cervical cancer.
The evidence of HPV DNA of high risk types in the plasma of women with CCs strongly emphasizes the necessity of more longitudinal comprehensive studies to determine its role as a possible biomarker in cervical cancer.
High risk human papillomavirus and Epstein Barr virus in human breast milk
The presence of high risk HPV and EBV in human milk suggests the possibility of milk transmission of these viruses, but given the rarity of viral associated malignancies in young people, it is possible but unlikely, that such transmission is associated with breast or other cancers.
High prevalence of human papillomaviruses in fresh frozen breast cancer samples
The high proportion of HPV positive breast cancers detected in this series using fresh frozen tissues cannot be dismissed, however the role of HPV in breast carcinogenesis remains unclear and may ultimately be ascertained by monitoring future breast cancer incidence amongst women vaccinated against high risk HPV types.
The blood DNA virome in 8,000 humans
The characterization of the blood virome is important for the safety of blood-derived transfusion products, and for the identification of emerging pathogens. We explored non-human sequence data from
No association between HPV positive breast cancer and expression of human papilloma viral transcripts
A large cohort of fresh frozen breast cancer and normal breast tissue specimens collected from patients in Australia for the presence of human papilloma virus DNA did not find any evidence for HPV or other viral transcripts in HPV DNA positive samples, suggesting that transcription of viral genomes is unlikely to be a significant factor in breast cancer pathogenesis.


Could Human Papillomaviruses Be Spread through Blood?
Examination of HPV DNA in banked, frozen peripheral blood mononuclear cells (PBMCs) from 57 U.S. pediatric patients collected between 1987 and 1996 and in fresh PBMCs from 19 healthy blood donors collected in 2002 to 2003 suggests that PB MCs may be HPV carriers and might spread the virus through blood.
Identification of human papillomavirus DNA gene sequences in human breast cancer
It is shown that HPV 18 gene sequences are present in DNA extracted from breast tumours in Australian women, and it is speculated that HPVs may be transmitted by hand from the female perineum to the breast.
General Acquisition of Human Papillomavirus Infections of Skin Occurs in Early Infancy
Skin swab samples from babies during their first days of life and from infants at various ages up to age 4 years suggest that asymptomatic HPV infections of normal skin are acquired very early in infancy and are caused by a great multiplicity of HPV types.
Detection of human papillomavirus types 16 and 18 mRNA in peripheral blood of advanced cervical cancer patients and its association with prognosis.
Results of this study seem to suggest that the presence of HPVE6 gene mRNA in peripheral blood may provide an early marker that identifies patients who are at risk for metastasis.
Detection and quantitation of human papillomavirus DNA in the plasma of patients with cervical carcinoma.
  • S. Dong, S. Pai, D. Sidransky
  • Medicine, Biology
    Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
  • 2002
Quantitative PCR identified the highest concentrations of HPV DNA (copy number of HPV/ml of plasma) in patients with invasive cervical cancer (mean, 11,163; median, 183), followed by a level of 8 in the single carcinoma in situ case and 0 copies in the normal control initially positive by conventional PCR.
Colorectal Papillomavirus Infection in Patients with Colorectal Cancer
The results suggest that colorectal HPV infection is common in patients with coloreCTal cancer, albeit at a low DNA copy number, with HPV16 being the most prevalent type.
A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin.
A pair of degenerate PCR primers was designed from two relatively conserved regions of the L1 open reading frame of most human papillomaviruses to be sensitive and generally applicable for detecting cutaneous HPV.
The Ubiquity and Impressive Genomic Diversity of Human Skin Papillomaviruses Suggest a Commensalic Nature of These Viruses
Swab samples collected from five different sites on the skin of renal transplant recipients, dialysis patients, and age- and sex-matched healthy controls were analyzed for HPV DNA by a newly designed PCR test, demonstrating that normal human skin harbors an array of papillomaviruses, most of them previously unknown.
Human papillomavirus is a necessary cause of invasive cervical cancer worldwide
The presence of HPV in virtually all cervical cancers implies the highest worldwide attributable fraction so far reported for a specific cause of any major human cancer, and the rationale for HPV testing in addition to, or even instead of, cervical cytology in routine cervical screening.