Human papillomavirus (HPV) type 6 and 16 DNA sequences in bronchial squamous cell carcinomas demonstrated by in situ DNA hybridization

  title={Human papillomavirus (HPV) type 6 and 16 DNA sequences in bronchial squamous cell carcinomas demonstrated by in situ DNA hybridization},
  author={Kari Juhani Syrj{\"a}nen and Stina M. Syrj{\"a}nen and Jari K. Kellokoski and Juhani K{\"a}rj{\"a} and Rauno M{\"a}ntyj{\"a}rvi},
A series of 131 routinely processed, paraffin-embedded biopsy specimens derived from the same number of patients with a bronchial squamous cell carcinoma were analyzed using in situ DNA-hybridization technique with a probe cocktail containing35S-labeled human papillomavirus (HPV) DNA of types 6, 11, 16, 18, and 30. The 12 carcinomas shown to contain HPV DNA by the probe cocktail were subjected to in situ hybridization with the specific HPV DNA probes applied separately under high stringency… 
Human papillomavirus type 11DNA in papillary squamous cell lung carcinoma
HPV type 11 may be associated with malignant conversion of benign papilloma of the pulmonary tract, as in the upper respiratory tract, and in situ hybridization with biotinylated probes is a relatively simple and appropriate method for retrospective analysis of HPV DNA sequences in surgical specimens.
Detection of oncogenic virus genomes and gene products in lung carcinoma
The data strongly suggest that the conventional human oncogenic viruses (HPV, EBV, HCMV, HHV-8 and SV40) are unlikely to play some role in the development of lung carcinomas.
Can HPV DNA testing on FNA material determine anogenital origin in metastatic squamous cell carcinoma?
Although reservations remain, particularly in relation to the prevalence of HPV DNA in non‐anogenital sites, HPV DNA testing by PCR can be successfully performed on FNA material, and can guide clinicians in assigning a site of origin.
Presence and activity of HPV in primary lung cancer
The results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells, therefore, the HPV is probably playing a role in lung carcinogenesis.
Detection of Human Papillomavirus in Bronchoalveolar Lavage Samples in Immunocompetent Children
This preliminary case-control study indicates the presence of HPV DNA in BAL samples in children and requires further investigation to elucidate the actual epidemiologic condition, the potential modes of its transmission, and its possible causative relationship in lung carcinogenesis in adulthood.
Detection of human papillomavirus genotypes in bronchial cancer using sensitive multimetrix assay.
Given the fact that initiation of smoking at an older age, fewer pack years and long smoking abstinence were associated with HPV, it is tempting to speculate that oncogenic HPV can substitute smoking as a risk factor, leading to lung cancer in these patients despite >22 years elapsing since their smoking cessation.
Human papillomavirus type 16 and 18 in primary lung cancers--a meta-analysis.
A meta-analysis to assess the prevalence of HPV16 and HPV18 in primary lung cancers found large heterogeneity across geographic regions and histological tissue types and further studies are needed to elucidate the role of HPV in lung carcinogenesis.
Human papillomavirus infections in lung cancer. Detection of E6 and E7 transcripts and review of the literature.
Data have been supported by the detection of E6 and E7 transcripts in HPV-positive lung cancer cases, reinforcing the hypothesis that oncogenic HPVs could act as cofactors in bronchial carcinogenesis.
Annual disease burden due to human papillomavirus 16 and 18 infections in Finland
  • K. Syrjänen
  • Biology, Medicine
    Scandinavian journal of infectious diseases. Supplementum
  • 2009
A systematic survey of the annual disease burden due to HPV6/11 infections in Finland was conducted and it was found that a minimum of 7859 to 8316 new cases of HPV16- or HPV18-associated clinical lesions would be detected each y in Finland, if all were registered.
Discovery of human papillomavirus in carcinoma of the lung.
It is difficult to comprehend that the success in linking HPVs as causative agents of cervical carcinoma has been heralded by thousands of articles and monographs, while less than two dozen scientific studies have been published that have addressed the role of HPVs in the etiology of pulmonary carcinoma.


Human papillomavirus DNA in squamous cell carcinomas of the larynx demonstrated by in situ DNA hybridization.
The present findings provide support to the concept on HPV as an etiological agent in laryngeal squamous cell carcinoma, most probably acting synergistically with chemical carcinogens.
Human papillomavirus type 11 DNA in squamous cell carcinomas and pre-existing multiple laryngeal papillomas.
Support is provided for the role of HPV as an etiological agent in laryngeal squamous cell carcinoma, most probably acting synergistically with chemical or physical carcinogens (one patient received irradiation and 2 others were smokers).
Detection of human papillomavirus (HPV) structural antigens and DNA types in inverted papillomas and squamous cell carcinomas of the nasal cavities and paranasal sinuses.
To assess the suggested etiological role of human papillomavirus (HPV), biopsies from 14 patients operated on for an inverted papilloma (11 cases) and squamous cell carcinoma (3 cases) of the nasal
Molecular cloning and characterization of the dna of a new human papillomavirus (hpv 30) from a laryngeal carcinoma
It is concluded that clone 4‐5 represents a new HPV type tentatively designated HPV 30, which was also detected in 2 genital lesions but not in 41 laryngeal carcinomas analyzed so far.
Human papillomavirus type 16 related DNA in an anaplastic carcinoma of the lung
One anaplastic carcinoma in the lung contained multiple copies of DNA hybridizing under stringent conditions to HPV 16 DNA, which originated from a 61‐year‐old female patient who underwent hysterectomy due to cervical cancer 9 years earlier.
A survey of human cancers for human papillomavirus DNA by filter hybridization
In addition to previous data demonstrating the association of HPV DNA with certain cancers of the skin and genital tract, data is presented that indicates that several additional human cancers also contain HPV‐related nucleotide sequences.
Rearranged HPV 16 molecules in an anal and in a laryngeal carcinoma
By hybridization under stringent conditions, one out of two anal carcinomas and one out of 36 laryngeal carcinomas were shown to harbor HPV 16 DNA in high copy number. Further analysis of both tumor