Human p53 and CDC2Hs genes combine to inhibit the proliferation of Saccharomyces cerevisiae.

@article{Nigro1992HumanPA,
  title={Human p53 and CDC2Hs genes combine to inhibit the proliferation of Saccharomyces cerevisiae.},
  author={Janice M. Nigro and Rachel P Sikorski and S. I. Reed and Bert Vogelstein},
  journal={Molecular and cellular biology},
  year={1992},
  volume={12 3},
  pages={1357-65}
}
Human wild-type and mutant p53 genes were expressed under the control of a galactose-inducible promoter in Saccharomyces cerevisiae. The growth rate of the yeast was reduced in cells expressing wild-type p53, whereas cells transformed with mutant p53 genes derived from human tumors were less affected. Coexpression of the normal p53 protein with the human cell cycle-regulated protein kinase CDC2Hs resulted in much more pronounced growth inhibition that for p53 alone. Cells expressing p53 and… CONTINUE READING