Human oocytes reprogram somatic cells to a pluripotent state

  title={Human oocytes reprogram somatic cells to a pluripotent state},
  author={Scott A. Noggle and Ho-Lim Fung and Athurva Jayavant Gore and Hector Martinez and K. C. Satriani and Robert W. Prosser and K. B Oum and Daniel Paull and Sarah Druckenmiller and Matthew Freeby and Ellen Greenberg and Kun Zhang and Robin Goland and Mark V. Sauer and Rudolph L. Leibel and Dieter Egli},
The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cells affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. Here we report that the development of human oocytes after genome exchange arrests at late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed… 

Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells

Improvements to the oocyte activation protocol are shown to promote development to the blastocyst stage, and diploid pluripotent stem-cell lines are derived from somatic cells of a newborn and, for the first time, an adult, a female with type 1 diabetes.

Reprogramming of two somatic nuclei in the same ooplasm leads to pluripotent embryonic stem cells

It is shown that the oocyte's reprogramming capacity is in excess of a single nucleus and that double nucleus‐transplanted embryos and derivative ESCs are very similar to their diploid counterparts.

Therapeutic Somatic Cell Reprogramming by Nuclear Transfer

An improved understanding of how the egg facilitates nuclear reprograming by natural means may identify components that can be used for more efficient reprogramming by this and other means.

Derivation and differentiation of haploid human embryonic stem cells

Diploidy is a fundamental genetic feature in mammals, in which haploid cells normally arise only as post-meiotic germ cells that serve to ensure a diploid genome upon fertilization. Gamete

Use of pluripotent stem cells for reproductive medicine: are we there yet?

Although the past years have yielded compelling information in understanding germ cell development via in vitro stem cell assays, extended investigative research is necessary in order to derive fully functional ‘artificial gametes’ in a safe way for future therapeutic applications.

Induction of pluripotency.

This chapter is a quasi-historical commentary of the various aforementioned approaches for the induction of pluripotency in lineage-committed cells, and introduces transcriptional and epigenetic changes occurring during reprogramming.

Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells

A recent study reported the generation of triploid human pluripotent stem cells by transferring somatic nuclei into oocytes, a variant form of SCNT.



Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells

It is established that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms.

Reprogramming of human somatic cells using human and animal oocytes.

The reprogramming of human somatic nuclei using oocytes obtained from animal and human sources is compared to call into question the potential use of these discordant animal oocyte sources to generate patient-specific stem cells.

Copy number variation and selection during reprogramming to pluripotency

It is shown that significantly more CNVs are present in early-passage human iPS cells than intermediate passage human i PS cells, fibroblast cell origins and with human embryonic stem (ES) cells.

Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development

The ability to create autologous embryos represents the first step towards generating immune-compatible stem cells that could be used to overcome the problem of immune rejection in regenerative medicine.

Development of Human Cloned Blastocysts Following Somatic Cell Nuclear Transfer with Adult Fibroblasts

It is demonstrated, for the first time, that SCNT can produce human blastocyst‐stage embryos using nuclei obtained from differentiated adult cells and provides new information on methods that may be needed for a higher level of efficiency for human nuclear transfer.

Epigenetic memory in induced pluripotent stem cells

It is observed that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbour residual DNA methylation signatures characteristic of their somatic tissue of origin, which favours their differentiation along lineages related to the donor cell, while restricting alternative cell fates.

Somatic coding mutations in human induced pluripotent stem cells

It is shown that 22 human induced pluripotent stem (hiPS) cell lines reprogrammed using five different methods each contained an average of five protein-coding point mutations in the regions sampled, and that hiPS cells acquire genetic modifications in addition to epigenetic modifications.

Persistent Donor Cell Gene Expression among Human Induced Pluripotent Stem Cells Contributes to Differences with Human Embryonic Stem Cells

It is reported that residual gene expression of the donor cell type contributes significantly to the differences among hiPSCs and hESCs, and adds to the incompleteness in reprogramming.