Human mucin 1 promoter drives human sodium/iodide symporter gene targeting expression in pancreatic carcinoma cells

@inproceedings{Qb2007HumanM1,
  title={Human mucin 1 promoter drives human sodium/iodide symporter gene targeting expression in pancreatic carcinoma cells},
  author={Zhou Qb and Chen Rf and Li Zh and Pan Qh and Zhou Jj and Tang Qb and Chen Js},
  year={2007}
}
Objective To clone human mucin 1 (MUC1) gene promoter and apply to drive human sodium/iodide symporter (hNIS) gene targeting expression in pancreatic carcinoma cells.Methods Human Mucinl (MUC1) promoter was cloned from the 5′ flanking region of the MUC1 gene by two-step nest PCR from human pancreatic carcinoma cells of the line CAPAN-Ⅰ,Ⅱ and then linked to pDC316 plasmid (pDC316-MUC1).Subsequently,a recombinant plasmid containing MUC1 and hNIS was constructed (pDC316-MUC1/hNIS).The recombinant… CONTINUE READING