Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE

  title={Human monocyte chemoattractant protein‐1 (MCP‐1) Full‐length cDNA cloning, expression in mitogen‐stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE},
  author={Teizo Yoshimura and Naoya Yuhki and Stephen K. Moore and Ettore Appella and Michael I. Lerman and Edward J. Leonard},
  journal={FEBS Letters},

Human monocyte chemotactic protein-2: cDNA cloning and regulated expression of mRNA in mesenchymal cells.

The regulated production of MCP-2 by tumor cells and normal mesenchymal cells is indicative of a role in neoplasia and inflammatory host responses.

Cloning of the gene for bovine monocyte chemoattractant protein-2.

An Amino acid sequence comparison of the bovine c11/1 gene product with human monocyte chemotactic proteins yielded the highest sequence identity with human MCP-2, and it is assumed that the c11-1-specific cDNA product represents the bivine M CP-2.

human monocyte chemoattractant protein-1 (MCP-1) : マウスJEのヒトhomologue

It is highly possible that MCP-1 is identical to JE and this protein may play an important role in macrophage infiltration in vivo.

The expression of monocyte chemotactic protein (MCP-1) in human vascular endotheliumin vitro andin vivo

Northern blot and slot blot analysis of RNA isolated from both the endothelium and the underlying vessel wall of freshly removed human arteries and veins showed MCP-1 transcripts, demonstrating for the first time that M CP-1 is expressed not only in atherosclerotic human arteries but also in symptom free artery and veins in vivo.

Functional role of the cis-acting elements in human monocyte chemotactic protein-1 gene in the regulation of its expression by phorbol ester in human glioblastoma cells

None of the MCP promoter constructs showed significant increase in CAT expression by treatment with tumor necrosis factor-α (TNF-α), which suggested that the TNF regulation of MCP-1 gene involves other parts of the gene besides the proximal 5′ flanking region.

Molecular Cloning of a Novel Human CC Chemokine Liver and Activation-regulated Chemokine (LARC) Expressed in Liver

This chemokine was mainly expressed in liver among various tissues and strongly induced in several human cell lines by phorbol myristate acetate and may represent a new group of CC chemokines localized on chromosome 2.

MCP‐1 is selectively expressed in the late phase by cytokine‐stimulated human neutrophils: TNF‐α plays a role in maximal MCP‐1 mRNA expression

Culture supernatants of phytohemagglutinin (PHA)‐stimulated human peripheral blood mononuclear cells (PHA‐sup) induced monocyte chemoattractant protein‐1 (MCP‐1) mRNA expression in human neutrophils.

Expression of a human mutant monocyte chemotactic protein 3 in Pichia pastoris and characterization as an MCP-3 receptor antagonist.

The cDNA encoding human monocyte chemotactic protein 3 was cloned in pHIL-S1, a vector designed for inducible secreted heterologous expression in the methylotrophic yeast Pichia pastoris to study antagonism of MCP-3 action in vitro and in disease models of cancer and inflammation.

Monocyte chemoattractant protein-1 gene is expressed in activated neutrophils and retinoic acid-induced human myeloid cell lines.

This is the first report that the MCP-1 gene is RA-responsive in myeloid cell lines and is expressed in neutrophils, which may play an important role in attracting monocytes to the site of tissue damage or infection.



Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties [published erratum appears in J Exp Med 1989 Dec 1;170(6):2189]

In the course of studies on cachectin/TNF being conducted in the laboratory, a novel macrophage product has been detected and characterized and appears to be an endogenous mediator of the inflammatory events induced by endotoxin.

Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines.

Another proinflammatory protein is purified that is chemotactic for human neutrophils from conditioned medium of lipopolysaccharide-stimulated monocytes and has up to 56% sequence similarity with several proteins that may be involved in host responses to infection or tissue injury.

A cDNA clone used to study mRNA inducible in human tonsillar lymphocytes by a tumor promoter.

Analysis of the pLD78 cDNA sequence revealed that it codes for a polypeptide consisting of 92 amino acid residues, including a putative signal sequence, which showed a significant homology with the corresponding regions of the human interleukin 2 and immune interferon genes.

Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.

A consensus sequence, comprised entirely of A and T residues and located in the 3'-untranslated region, is conserved in toto in the murine and human TNF mRNAs, suggesting that it may serve a specific regulatory function among the m RNAs in which it is found.

Cloning and characterization of a novel T cell activation gene.

The combined data suggest that TCA3 may represent a new lymphokine, a T cell gene selectively expressed during activation via the antigen-receptor pathway, which is not transcribed in response to interleukin 2 stimulation, but can be detected as early as 1 hr poststimulation.

Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties

The identification and purification of a new inflammatory monokine synthesized by the macrophage tumor cell line RAW 264.7 suggest that MIP is an endogenous mediator that may play a role in the host responses that occur during endotoxemia and other inflammatory events.

A human T cell-specific molecule is a member of a new gene family.

There is significant homology between the RANTES sequence and several other T cell genes, suggesting that they comprise a previously undescribed family of small T cell molecules.

Purification of a monocyte chemotactic factor secreted by nonhuman primate vascular cells in culture.

A dose-dependent relationship was observed between SMC-CF concentration and monocyte chemotactic activity, with maximal and half-maximal biologic activity being observed at approximately 5 and 0.1 nM, respectively.

Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties.

Structural and regulatory features of the JE gene and its product that link it to a family of cytokines, including macrophage colony-stimulating factor, interferon alpha, interleukin 6, and interLEukin 2 are presented.