Human monocyte carboxylesterase. Purification and kinetics.

  title={Human monocyte carboxylesterase. Purification and kinetics.},
  author={A. M. Saboori and D. Newcombe},
  journal={The Journal of biological chemistry},
  volume={265 32},
  • A. M. Saboori, D. Newcombe
  • Published 1990
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • Human peripheral blood monocytes were isolated by density gradient centrifugation and purified by counterflow centrifugation elutriation. Membrane-localized carboxylesterase (CBE) was extracted with nonionic detergent (Triton X-100) and purified by ion exchange (DEAE-cellulose), gel filtration (Sephacryl S-300), hydroxylapatite column, and high performance liquid chromatography. The purified enzyme migrated on 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single protein… CONTINUE READING
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