Human monoclonal antibody derived from an autoimmune thrombocytopenic purpura patient, recognizing an intermediate filament's determinant common to vimentin and desmin.

@article{Varon1990HumanMA,
  title={Human monoclonal antibody derived from an autoimmune thrombocytopenic purpura patient, recognizing an intermediate filament's determinant common to vimentin and desmin.},
  author={David Varon and Stig Linder and E Gembom and L Guedg and H Langbeheim and Alain Berrebi and Zelig Eshhar},
  journal={Clinical immunology and immunopathology},
  year={1990},
  volume={54 3},
  pages={454-68}
}
Human monoclonal antibody (mAb) technology has been helpful in identifying autoantibodies that are involved in various autoimmune disorders. We report here the results of such an attempt to immortalize antibody-forming cells from spleen of an autoimmune thrombocytopenic purpura (ATP) patient and characterize the resulting mAb. The human mAb we derived, denoted (4G9), binds to the cytoskeletal network. Using immunofluorescence analyses of permeabilized and fixed cell lines and tissues, the 4G9… CONTINUE READING