Human monoclonal antibodies

@article{Cole2004HumanMA,
  title={Human monoclonal antibodies},
  author={Susan P. C. Cole and Barbara G Campling and Tsehay Atlaw and Danuta Kozbor and John C. Roder},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={62},
  pages={109-120}
}
SummaryThe technology for the production of murine monoclonal antibodies has been refined enormously since its introduction in 1975. However, the technology for generating human monoclonal antibodies has only recently come into its own. In this review, three currently available approaches to the production of human monoclonal antibodies are described. These include the hybridoma technique, based on the fusion of antibody-producing human B lymphocytes with either mouse or human myeloma or… Expand
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This review serves as an introduction to the immortalization of antigen-specific human B cell and hybridoma technologies, phage display platform, the use of transgenic mice, and the generation of monoclonal antibodies from single B cells. Expand
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An overview of phage display derived recombinant antibodies against bacterial, viral and eukaryotic pathogens, as well as microbial toxins, intended for diagnostic and therapeutic applications are presented. Expand
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The use of human monoclonal autoantibodies are described to characterize an activation specific antigen on GPIIIa and an autoantigen on the GPIb complex to determine whether these autoantibia emerge from a pool of naturally occurring antibodies to activation or senescence antigens, or are triggered by environmental agents such as bacteria or virus. Expand
Development of whole-porcine monoclonal antibodies with potent neutralization activity against classical swine fever virus (CSFV) from single B cells
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The results suggest that the results not only provide a method for efficiently obtaining mAbs against CSFV but also offer promising mAb candidates for development of antibody-based diagnostic and antiviral agents, and it is demonstrated that these two mAbs can be used as novel reagents for detecting virus infection. Expand
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Adapt vectors for gene transfer at each stage of differentiation from CD34+ cells to thymocytes and mature T cells are proposed to allow some new clinical protocols in gene therapy with a co-transplantation of genetically modified stem cells and their differentiated T progenitors in order to reduce the aplasia stage induced by current transplantation protocols. Expand
Antibody-drug conjugates for cancer therapy: The technological and regulatory challenges of developing drug-biologic hybrids.
  • G. S. Hamilton
  • Medicine
  • Biologicals : journal of the International Association of Biological Standardization
  • 2015
TLDR
The combination of a mAb targeting a cancer-specific antigen with a cytotoxin has tremendous promise as a new type of targeted cancer therapy. Expand
Immunoscintigraphy and radioimmunotherapy in Cuba: experiences with labeled monoclonal antibodies for cancer diagnosis and treatment (1993-2013).
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Evidence obtained suggests promising potential of monoclonal antibodies and nuclear medicine, with immunoscintigraphy and radioimmunotherapy techniques providing alternatives for cancer diagnosis and treatment in Cuba. Expand
Improving scFv stability through framework engineering
The availability of cost-effective high throughput screening assays combined with an enhanced understanding of oncogenesis has driven the development of more potent, specific, and less toxic… Expand
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