Human monoclonal antibodies

@article{Cole2004HumanMA,
  title={Human monoclonal antibodies},
  author={Susan P. C. Cole and Barbara G Campling and Tsehay Atlaw and Danuta Kozbor and John C. Roder},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={62},
  pages={109-120}
}
SummaryThe technology for the production of murine monoclonal antibodies has been refined enormously since its introduction in 1975. However, the technology for generating human monoclonal antibodies has only recently come into its own. In this review, three currently available approaches to the production of human monoclonal antibodies are described. These include the hybridoma technique, based on the fusion of antibody-producing human B lymphocytes with either mouse or human myeloma or… 

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References

SHOWING 1-10 OF 161 REFERENCES

Human monoclonal antibody against Forssman antigen.

Findings point to the potential use of human-mouse hybrid cells as a source of human monoclonal antibodies for therapeutic and diagnostic purposes.

Production of human hybridomas secreting antibodies to measles virus

A hypoxanthine phosphoribosyl transferase (HPRT)-deficient human B-cell line derived from a patient suffering from multiple myeloma with peripheral lymphocytes obtained from a patients with subacute sclerosing panencephalitis were found to secrete human IgM specific for measles virus nucleocapsids.

Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes

The continuous production in vitro of a monoclonal IgM anti-IgG antibody (rheumatoid factor, r.f.) by a lymphoblastoid cell line established from a patient with rheumatoids arthritis is demonstrated.

Antibody producing human-human hybridomas. II. Derivation and characterization of an antibody specific for human leukemia cells

The study demonstrates that some patients with AML generate an immune response against their autologous malignant cells, and that the antigenic determinant in the case of aml-18 is also expressed specifically on leukemic cells from other patients.

Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.

  • L. OlssonH. Kaplan
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
It is reported the establishment of human-human hybridomas producing monoclonal antibody of predefined antigenic specificity from patients with untreated Hodgkin's disease who had been previously sensitized to the chemical allergen 2,4-dinitrochlorobenzne.

Determination of the optimal human cell lines for development of human hybridomas.

Among the lines tested, UC729-6 and HF2 appeared optimal for pursuing further studies with human-human hybridomas, and although only a small percentage of hybrids were produced with HMy2, a very high percentage secreted immunoglobulin, so that this line also warrants further investigation to improve the efficiency of hybrid formation.

Generation of human monoclonal antibodies reactive with human mammary carcinoma cells.

It is demonstrated that stable clones of human-mouse hybridomas can be generated by using lymph nodes of mastectomy patients and that clones can be selected which synthesize human monoclonal antibodies reactive with human mammary carcinoma cells.
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