Human mitochondrial thioredoxin reductase cDNA cloning, expression and genomic organization.

@article{MirandaVizuete1999HumanMT,
  title={Human mitochondrial thioredoxin reductase cDNA cloning, expression and genomic organization.},
  author={Antonio Miranda-Vizuete and Anastasios E. Damdimopoulos and Jos{\'e} Rafael Pedrajas and J A Gustafsson and Giannis Spyrou},
  journal={European journal of biochemistry},
  year={1999},
  volume={261 2},
  pages={405-12}
}
We have isolated a 1918-bp cDNA from a human adrenal cDNA library which encodes a novel thioredoxin reductase (TrxR2) of 521 amino acid residues with a calculated molecular mass of 56.2 kDa. It is highly homologous to the previously described cytosolic enzyme (TrxR1), including the conserved active site CVNVGC and the FAD-binding and NADPH-binding domains. However, human TrxR2 differs from human TrxR1 by the presence of a 33-amino acid extension at the N-terminus which has properties… CONTINUE READING